Are multiplexed metabarcoding panels comparable to individual marker gene library preparations?

Analysis of multiple marker genes using metabarcoding environmental DNA (eDNA) can offer information greater than that from sequencing single genes, such as responses across the phylogenetic tree to gradients (Cordier et al. 2019). Furthermore, regions same gene be sequenced improve resolution (Fuks 2018). However, separate amplification reactions and library preparation steps for each costly time consuming. Here, we have designed optimised a multiplex panel four (two 18S rRNA gene, one region 16S rbcL gene). By combining into reaction, labwork required is decreased, reducing cost time. This compared with widely available commercial microbial (bacterial fungal) screening individual preparations gene.